Coding

Part:BBa_K1074009:Design

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-16)


SdpA,SdpB,SpbC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
    Illegal AgeI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746


Design Notes

With restriction sites of Spe I and Pst I in the sequence.


Source

From the genome of Bacillus subtilis (strain 168)

References